Prevalence, Genome Characterization, and Phylogenetic Analysis of Bovine Hepacivirus in Inner Mongolia, Northeastern China

Veterinary Medicine
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Ziyan Liu# Center for Pathogen Biology and Infectious Diseases, International Center of Future Science, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital, Jilin University, Changchun 130122, Jilin, People’s Republic of China. Laboratory of Pathogen Microbiology and Immunology, College of Life Science, Jilin Agricultural University, Changchun, 130122, Jilin, People’s Republic of China

Liang Li# Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130122, Jilin, People’s Republic of China

Guo Yu# Center for Animal Disease Prevention and Control of Inner Mongolia, Hohhot 010010, Inner Mongolia, People’s Republic of China

Wenbo Xu Center for Pathogen Biology and Infectious Diseases, International Center of Future Science, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital, Jilin University, Changchun 130122, Jilin, People’s Republic of China

Yongxu Yuan Laboratory of Pathogen Microbiology and Immunology, College of Life Science, Jilin Agricultural University, Changchun, 130122, Jilin, People’s Republic of China

Xiaojie Liang Laboratory of Pathogen Microbiology and Immunology, College of Life Science, Jilin Agricultural University, Changchun, 130122, Jilin, People’s Republic of China

Wei Wang Center for Animal Disease Prevention and Control of Hulunbuir, Hulunbuir 021000, Inner Mongolia, People’s Republic of China

Yinghua Zhao Center for Pathogen Biology and Infectious Diseases, International Center of Future Science, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital, Jilin University, Changchun 130122, Jilin, People’s Republic of China

Liyan Sui Center for Pathogen Biology and Infectious Diseases, International Center of Future Science, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital, Jilin University, Changchun 130122, Jilin, People’s Republic of China

Xianmin Feng Academy of Basic Medicine, Jilin Medical University, Jilin 132013, Jilin, People's Republic of China

Feng Wei* Laboratory of Pathogen Microbiology and Immunology, College of Life Science, Jilin Agricultural University, Changchun, 130122, Jilin, People’s Republic of China

Quan Liu* Center for Pathogen Biology and Infectious Diseases, International Center of Future Science, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital, Jilin University, Changchun 130122, Jilin, People’s Republic of China. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130122, Jilin, People’s Republic of China. Laboratory of Emerging Vector‐borne diseases, School of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong, People’s Republic of China.

Zedong Wang* Center for Pathogen Biology and Infectious Diseases, International Center of Future Science, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital, Jilin University, Changchun 130122, Jilin, People’s Republic of China. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130122, Jilin, People’s Republic of China.

# contributed equally to this work


Abstract

Objective: Bovine hepacivirus (BovHepV) is a new member of the genus Hepacivirus in the family Flaviviridae, which has been detected in cattle in more than seven countries. The purpose of the study was to identify and genetically characterize BovHepV in cattle in northeastern China. 

Methods: A total of 116 serum samples from free-range cattle were collected from HulunBuir city in April and May, 2021, and were divided into three pools before being subjected to metagenomic sequencing. The BovHepV sequences obtained were used for genome characterization and phylogenetic analysis. 

Results: Two 8840 nucleotides long BovHepV strains YKS01/02 were identified in the samples through metagenomic sequencing. In RT-PCR screening, the prevalence of BovHepV was 8.8% (5/57) in Yakeshi of HulunBuir. The novel virus showed a range of 79.3%–91.9% nucleotide sequence identity and 92.5%–98.1% amino acid sequence identity with the BovHepV sequences discovered. Phylogenetic analysis classified YKS01/02 strains into BovHepV subtype G strains discovered in Jiangsu Province, China, and named with subtype G2. 

Conclusion: This study first identified BovHepV in cattle in northeastern China, and expands the geographical distribution and genetic diversity of BovHepV in the country.

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