A cloning and functional study of MYB upstream lncRNA

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chao liu#
Shanghai Ocean University
Shanghai Ocean University
Bingshe Han*
Shanghai Ocean University
Shanghai Ocean University

# contributed equally to this work, * Corresponding author


The proto-oncogene MYB is an important transcription factor, which plays an important
role in the proliferation and differentiation of hematopoietic cells. Although MYB has been well
studied, the detailed mechanism of MYB regulation still remains unclear. The aim of this study is to
explore the effects of a lncRNA, which was transcribed from the upstream region of MYB, on MYB
expression and the proliferation of K562 cells. The full length of the above lncRNA was obtained
by rapid amplification of cDNA ends (RACE). The effects of the lncRNA on the proliferation,
invasion, and migration of K562 cells were examined in vitro using the Cell Counting Kit 8 (CCK8)
and Transwell. The results indicated that the overexpression of the lncRNA promotes the mRNA
and protein levels of the MYB gene in K562 cells, and knockdown of the lncRNA decreases the
expression of MYB. In addition, lncRNA overexpression promotes the growth of K562 cells, and
knockdown of the lncRNA significantly inhibits the proliferation, migration and invasion of K562
cells. Based on our data, we conclude that this lncRNA, which is transcribed by the upstream of the
MYB gene, can be used as a molecular markers or target for tumor diagnosis and treatment,
especially in leukemia. More specifically, the above lncRNA can be applied to the detection and
treatment and modern drug development of leukemia, which has far-reaching clinical significance.

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  • 25 Oct 2023 21:06 Version 1
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