Regulation of the proto-oncogene MYB by long non-coding RNA in leukemia

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Mengjie Shi#
Shanghai Ocean University
Shanghai Ocean University
Bingshe Han*
Shanghai Ocean University
Shanghai Ocean University

# contributed equally to this work, * Corresponding author


The transcription factor MYB plays vital roles in regulating proliferation
and differentiation of hematopoietic progenitor cells, dysregulation of MYB has been
implicated in the pathogenesis of leukemia. Although the transcription of MYB has
been well studied, its detailed underlying regulatory mechanisms still remain elusive.
Our study found that a long non-coding RNA (lncRNA) was transcribed from the -96k
region of the MYB gene, and the full length was obtained by rapid-amplification of
cDNA ends (RACE). Then, overexpression and knockdown vectors of lncRNA were
constructed,we found overexpression of lncRNA can promote the transcription and
protein synthesis of the MYB gene in K562 cells, and proliferation, migration and
invasion of K562 cells. However, MYB was downregulated through inhibiting
expression of lncRNA, and significantly inhibit the proliferation, migration and
invasion of K562 cells. Taken together, our data revealed that -96kb lncRNA was
required for MYB expression and was transcribed to produce lnRNAs, playing
important roles in leukemia initiation and progression.

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  • 25 Oct 2023 20:57 Version 1
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